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 ที่มา:Kasetsart Journal (Natural Science)(วารสารวิทยาสารเกษตรศาสตร์ สาขาวิทยาศาสตร์)หัวเรื่อง:Characterization of antibacterial protein from hemolymph of oyster Crassostrea belcheri |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of Antibacterial Protein from Hemolymph
of Oyster Crassostrea belcheri) ผู้เขียน: วิราวรรณ จุลโพธิ์ (นุชนารถ),  ดร.สุภาวดี พุ่มพวง, ศาสตราจารย์ , ดร.สุริยัน ธัญกิจจานุกิจ, ผู้ช่วยศาสตราจารย์ , ดร.สุตเขตต์ นาคะเสถียร, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe hemolymph of oyster Crassostrea belcheri was found to possess significant antibacterial
activity against Vibrio harveyi, V. vulnificus and V. cholerae. Hemolymph was partially purified by gel
filtration chromatography on Sephacryl S-200 and fractioned into four parts: P1, P2, P3 and P4. The P3
showed the highest antibacterial activity against V. parahaemolyticus, V. cholerae, V. alginolyticus, V.
harveyi in the inhibition ranges from 95-62% and V. vulnificus was inhibited only 8% with 64 mg
protein. The antibacterial activity of P3 protein was stabilized at pH 6-8 and 4-30?C. In addition, the
activity required calcium ion. Molecular weight of the antibacterial protein P3 determined by gel filtration
was approximately 18.7 kDa. Antibacterial protein gave two bands of 25.0 and 30.5 kDa in SDS-PAGE
and pI was 3.0 and 5.0, respectively. Both bands were analyzed by LC-MS/MS and compared with
nrFasta database. The 30.5 kDa band was homologous to hemocyte extracellular superoxide dismutase
from C. gigas and 25.0 kDa band was homologous to sarcoplasmic calcium-binding protein. |
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